The lens tissue GSH and LPO levels were determined according to the method followed by Beutler 10 and Ledwozyw et al., respectively. The homogenates were used for protein, glutathione (GSH), lipid peroxidation (LPO) levels, and enzymes activities. The homogenates were centrifuged, and the clear supernatant was used. The tissues were homogenized in cold saline using a glass homogenizer to make 10% (w/v) homogenate. Lens tissue samples were taken and frozen until needed for study. This study showed that Vit U exerted antioxidant properties and may prevent lens damage caused by VPA. Treatment with Vit U reversed these effects. On the other hand, glutathione levels, superoxide dismutase, glutathione peroxidase, glutathione reductase, glutathione- S-transferase, and paraoxonase activities were decreased in VPA groups. Lens lipid peroxidation levels and aldose reductase and sorbitol dehydrogenase activities were increased in VPA group. The homogenates were used for protein, glutathione, lipid peroxidation levels, and antioxidant enzymes activities. Lens was taken from animals, homogenized in 0.9% saline to make up to 10% (w/v) homogenate. On the 16th day of experiment, all the animals which were fasted overnight were killed. Vit U was given to rats by gavage and VPA was given intraperitoneally. Group IV was given VPA + Vit U (in same dose and time). Group III was given only VPA (500 mg/kg/day) for 15 days. Group II included control rats supplemented with Vit U (50 mg/kg/day) for 15 days. Female Sprague Dawley rats were divided into four groups. This study was aimed to investigate the effects of Vit U on lens damage parameters of rats exposed to VPA. S-Methylmethioninesulfonium is a derivative of the amino acid methionine and it is widely referred to as vitamin U (Vit U). VPA exhibits various side effects such as organ toxicity, teratogenicity, and visual disturbances. Valproic acid (2-propyl-pentanoic acid, VPA) is the most widely prescribed antiepileptic drug due to its ability to treat a broad spectrum of seizure types. All subjects Allied Health Cardiology & Cardiovascular Medicine Dentistry Emergency Medicine & Critical Care Endocrinology & Metabolism Environmental Science General Medicine Geriatrics Infectious Diseases Medico-legal Neurology Nursing Nutrition Obstetrics & Gynecology Oncology Orthopaedics & Sports Medicine Otolaryngology Palliative Medicine & Chronic Care Pediatrics Pharmacology & Toxicology Psychiatry & Psychology Public Health Pulmonary & Respiratory Medicine Radiology Research Methods & Evaluation Rheumatology Surgery Tropical Medicine Veterinary Medicine Cell Biology Clinical Biochemistry Environmental Science Life Sciences Neuroscience Pharmacology & Toxicology Biomedical Engineering Engineering & Computing Environmental Engineering Materials Science Anthropology & Archaeology Communication & Media Studies Criminology & Criminal Justice Cultural Studies Economics & Development Education Environmental Studies Ethnic Studies Family Studies Gender Studies Geography Gerontology & Aging Group Studies History Information Science Interpersonal Violence Language & Linguistics Law Management & Organization Studies Marketing & Hospitality Music Peace Studies & Conflict Resolution Philosophy Politics & International Relations Psychoanalysis Psychology & Counseling Public Administration Regional Studies Religion Research Methods & Evaluation Science & Society Studies Social Work & Social Policy Sociology Special Education Urban Studies & Planning BROWSE JOURNALS
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